Background:

Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC.

Applications:

ELISA, WB

Name of antibody:

PPP1R12A

Immunogen:

Fusion protein of human PPP1R12A

Full name:

protein phosphatase 1, regulatory subunit 12A

Synonyms：

MBS; M130; MYPT1

SwissProt:

O14974

ELISA Recommended dilution:

1000-2000

WB Predicted band size:

115 kDa

WB Positive control:

A172 cells

WB Recommended dilution:

200-1000