Background:

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Applications:

WB, IHC

Name of antibody:

PAK1(Phospho-Ser199)

Immunogen:

Peptide sequence around phosphorylation site of Serine 199(T-K-S(p)-V-I) derived from Human PAK1.

Full name:

p21 protein (Cdc42/Rac)-activated kinase 1

Synonyms：

PAKalpha

SwissProt:

Q13153

IHC positive control:

Human placenta tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

61 kDa

WB Positive control:

LOVO cells lysates treated with starved

WB Recommended dilution:

500-1000