Background:

The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum.

Applications:

WB, IHC, IF

Name of antibody:

MID1

Immunogen:

Synthesized peptide derived from internal of human TRI18.

Full name:

midline 1

Synonyms：

OS; FXY; OSX; OGS1; XPRF; BBBG1; GBBB1; MIDIN; RNF59; ZNFXY; TRIM18

SwissProt:

O15344

IHC positive control:

Human brain tissue

IHC Recommend dilution:

50-100

WB Predicted band size:

75 kDa

WB Positive control:

293 cells lysate

WB Recommended dilution:

500-3000

IF positive control:

Hela cells

IF Recommend dilution:

100-500