Background:

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Applications:

ELISA, WB, IHC

Name of antibody:

PCNA

Immunogen:

Fusion protein of human PCNA

Full name:

proliferating cell nuclear antigen

Synonyms:

ATLD2

SwissProt:

P12004

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human prostate cancer and human brain

IHC Recommend dilution:

25-100

WB Predicted band size:

29 kDa

WB Positive control:

293T and HEPG2 cell lysates

WB Recommended dilution:

500-2000