Background:

proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

Applications:

WB, IHC, IF

Name of antibody:

SRC (Phospho-Tyr529)

Immunogen:

Synthetic peptide of human SRC (Phospho-Tyr529)

Full name:

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (Phospho-Tyr529)

Synonyms：

ASV; SRC1; c-SRC; p60-Src

SwissProt:

P12931

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

60 kDa

WB Positive control:

HT29 cells untreated or treated with PMA

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200