Background:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for this gene. A pseudogene has been identified on the Y chromosome.

Applications:

ELISA, WB, IHC

Name of antibody:

PSMA6

Immunogen:

Fusion protein of human PSMA6

Full name:

proteasome subunit alpha 6

Synonyms:

IOTA; p27K; PROS27

SwissProt:

P60900

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human cervical cancer

IHC Recommend dilution:

100-300

WB Predicted band size:

27 kDa

WB Positive control:

A375, HL60 cell, Mouse skeletal muscle tissue, Jurkat, 293T cell lysates

WB Recommended dilution:

1000-5000