Background:

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]

Applications:

WB

Name of antibody:

TUBA1/3/4(Phospho-Tyr272)

Immunogen:

Peptide sequence around phosphorylation site of tyrosine 272 (A-T-Y(p)-A-P) derived from Human TUBA1/3/4.

Full name:

tubulin, alpha 1a/tubulin, alpha 1b/tubulin, alpha 1c/tubulin, alpha 3c/tubulin, alpha 3e/tubulin, alpha 4a

Synonyms：

LIS3; TUBA3; B-ALPHA-1/K-ALPHA-1/TUBA6; bcm948/TUBA2; bA408E5.3/TUBA1; H2-ALPHA

SwissProt:

Q71U36/P68363/Q9BQE3/Q13748/Q6PEY2/P68366

WB Predicted band size:

50/ kDa

WB Positive control:

Rat brain tissue lysates

WB Recommended dilution:

500-1000